VEGF-A, Blocking Antibody Anti-Mouse 100 µg
Description / VEGF-A, Blocking Antibody
Mouse VEGF (or VEGF-A) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains. The antibody blocks the binding of VEGF to VEGFR-2 ( mouse FLK) and therefore the initiation of signal transduction.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB (mouse/human); Neutralization of VEGF: human, mouse |
| Clone Nr. | (#MAB0705) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with purified mouse VEGF). |
| Antigen | recombinant mouse VEGF |
| Application | N, WB |
| Synonyms | Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188 |
| Uniprot ID | Q00731 |
| Protein RefSeq | NP_001020421 |
| mRNA RefSeq | NM_001025250 |