Protocols from Researchers
The following protocols are from researchers using ReliaTech's antibodies.
Immunohistochemistry (IHC)
Epitope demasking
Demasking of epitopes in paraffin sections
- Tissue fixation and preparation (PDF, German)
- Target Retrieval-Puffer (PDF, German)
Heat treatments
- within the autoclave (PDF, German)
- within the microwave (PDF, German)
- within the pressure cooker (PDF, German)
- with water bath (PDF, German)
- with steam (PDF, German)
Proteolytic Treatments
- Proteolytische Vorbehandlung (PDF, German)
- Kombinantion von Methoden (PDF, German)
Immunofluorescence (IF)
Immunofluorescence with lymphatic endothelial cells (LECs)
a) Protocol for immunofluorescence with lympathic endothelial cells (LEC) using Lab-Tek II Chamber Slides. As primary antibodies anti-human LYVE-1 (ReliaTech Cat# 102-PA50), anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).
b) Protocol for immunofluorescence with lympathic endothelial cells (LEC) and HUVECs in a cell culture dish. As primary antibodies anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).
Flow Cytometry
FACS analysis with human macro- / microvascular endothelial cells
a) HUVEC / HDMVEC (German / English)
Bioassays
a) Thymidine incorporation assay for primary human endothelial cells (PDF, English)
b) Thymidine incorporation assay for Balb/c 3T3 cells (German / English)