Description / VEGF-A
Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains.
More Information
| Size | 50 µg |
|---|---|
| Source | Rabbit |
| Biological Activity | ELISA: use at 1-10 µg/ml. |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse, Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant murine VEGF164. Anti-murine VEGF-A was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant mouse VEGF164 (RT #M30-001) |
| Application | E |
| Synonyms | Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188 |
| Uniprot ID | Q00731 |
| Protein RefSeq | NP_001020421 |
| mRNA RefSeq | NM_001025250 |