Description / TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a member of the TNF superfamily. Human OX40L shares 46% amino acid sequence identity with its mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similar to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily, which is expressed predominantly on activated CD4+ T cells.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | FC: 1:50-100; IHC (frozen): 1:20-200; Neutralization: In the presence of 10 ng/ml recombinant human OX40, ED50 of mouse anti-human Ox40L (101-M681; #8S23) is 0.5-1.5 µg/ml. --> The dose of the neutralization assay is for reference only and the final dose |
| Clone Nr. | (#8S23) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody war produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human TNFSF4, also called Ox40 ligand. The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Recombinant human TNFSF4 |
| Application | IHC (F), FC, N |
| Synonyms | TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; Ox 40 Ligand |
| Uniprot ID | P23510 |
| Protein RefSeq | NP_003317.1 |
| mRNA RefSeq | NM_003326.3 |