Description / TIE-2
Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB: 1:500-1000; ELISA: 1:200-400; IHC: 1:50 |
| Clone Nr. | (#21G7) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human extracellular domain of TIE-2 fragment). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity c |
| Antigen | human Tie2 extracellular domain |
| Application | ELISA, WB, IHC |
| Synonyms | TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B |
| Uniprot ID | Q02763 |
| Protein RefSeq | NP_000450.2 |
| mRNA RefSeq | NM_000459.3 |