Mouse Gremlin-1
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| Cat-Nr. | M30-025 |
| Size | 50 µg |
| Price | 185 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 161 |
| Molecular Weight | 20.7 kDa |
| N Terminal Sequence | MKKKGSQGAIPP |
| Biological Activity | Data not available. |
| Species Reactivity | Mouse |
| Buffer | 50 mM acetic acid |
| Reconstitution | Mouse Grem-1 should be reconstituted in 50 mM acetic acid or water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C. |
| Stability and Storage | The lyophilized mouse Grem-1, though stable at room temperature, is best stored desiccated below 0°C |
| Synonyms | Grem1; ld; Drm; Cktsf1b1 |
| Description | Gremlin was identified in a Xenopus expression cloning screen as a dorsalizing factor that can induce a secondary axis. A rat homolog, called Drm, was identified as a cDNA that was down regulated in v Mos transfected cells. Gremlin/Drm belongs to the DAN family of secreted glycoproteins that are BMP antagonists. Other members of the family include: Cerberus, Dante, PRDC, Caronte and DAN. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGFβ superfamily ligands. In vitro, Gremlin/Drm binds BMP4 and BMP2 indicating that it might interfere with BMP signaling. Gremlin/Drm acts as a BMP2/ 4 antagonist in a variety of tissues and developmental processes including: Xenopus animal cap explants, chick limb bud outgrowth and chondrogenesis, murine lung branching morphogenesis, and osteogenic differentiation of mouse myoblasts and bone marrow stromal cells. In addition, expression of Gremlin/Drm has been shown to be down-regulated in a wide range of human cancer cell lines. Mouse, human, chick and Xenopus homologs of Gremlin share over 80% amino acid identity. It is likely that various DAN family members and other BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGFβ superfamily ligands. |
| Protein Sequence | MKKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD |
| Uniprot ID | O70326 |
| Protein RefSeq | NP_035954.1 |
| mRNA RefSeq | NM_011824.4 |
Figures
SDS-PAGE analysis of recombinant mouse Gremlin-1. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1: Molecular weight marker (kDa); Lane 2: 500ng Gremlin-1; Lane 3: 1000ng Gremlin-1.
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