Human PDGF-BB
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| Cat-Nr. | 200-055 |
| Size | 5 µg |
| Price | 85 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 109 |
| Molecular Weight | 24.3 kDa |
| N Terminal Sequence | SLGSL |
| Endotoxin Levels | < 0.1 ng per ug of PDGF-BB |
| Biological Activity | The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts). |
| Species Reactivity | Human |
| Buffer | 50mM acetic acid |
| Reconstitution | Centrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50mM acetic acid to a concentration not lower than 100μg/ml. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA; 0.1%) is recommended. |
| Stability and Storage | The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-BB is best stored at -20°C to -70°C. |
| Synonyms | PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN=Becaplermin |
| Description | PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids). |
| Protein Sequence | SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT |
| Uniprot ID | P01127 |
| Protein RefSeq | NP_002599.1 |
| mRNA RefSeq | NM_002608.2 |
Figures
SDS-PAGE analysis of recombinant human PDGF-BB. Sample was loaded in 15% SDS-polyacrylamide gel under non-reducing condition and stained with Coomassie Blue.
Proliferation assay with NHDF cells. The cells were stimulated using recombinant human PDGF-BB and the WHO standard 74/728. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
Reference
- CD146+ Pericytes Subset Isolated from Human Micro-Fragmented Fat Tissue Display a Strong Interaction with Endothelial Cells: A Potential Cell Target for Therapeutic Angiogenesis. E. Manocha et al., Int J Mol Sci. 2022 May; 23(10): 5806.
- Extracellular Vesicles Mediate Communication between Endothelial and Vascular Smooth Muscle Cells. M. Fontaine et al., Int J Mol Sci. 2022 Jan; 23(1): 331.
- Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy. Rezzola S. et al., Diabetes. 2015 Jul;64(7):2581-92.
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