Human Dkk-2
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| Cat-Nr. | 200-014 |
| Size | 20 µg |
| Price | 185 € |
| Source | E. coli |
| Label | His-Tag |
| Formulation | lyophilized from PBS |
| Purity Confirmation | 95% by SDS-PAGE & visualized Coomassie stain |
| Length [aa] | 235 |
| Molecular Weight | 26.0 kDa |
| Species Reactivity | Human |
| Buffer | PBS |
| Synonyms | Dickkopf-related protein-2, Dickkopf-2 |
| Description | The dickkopf (DKK)-related protein family is comprised of four central members, DKK-1 - 4, along with the distantly-related DKK family member DKK-11 (Soggy), which is thought to be a descendent of an ancestral DKK-3 precursor due to its unique sequence homology to DKK-3 and no other DKK family member. DKK family members, with the exception of the divergent Soggy, share two conserved cysteine-rich domains and show very little sequence similarity outside of these domains. Playing an important regulatory role in vertebrate development through localized inhibition of Wnt-regulated processes, including anterior-posterior axial patterning, limb development, somitogenesis, and eye formation, DKKs have also been implicated post-developmentally in bone formation, bone disease, cancer, and neurodegenerative diseases. DKK proteins typically play an important regulatory role in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/β-catenin signaling cascade. DKK-2 has been shown to both inhibit and enhance canonical Wnt signaling; enhancing Wnt signaling through direct high-affinity binding of DKK-2 to LRP6 during LRP6 overexpression, while inhibiting Wnt signaling and promoting LRP6 internalization through the formation of a ternary complex between DKK-2, LRP6, and Kremen-2. Recombinant Human DKK-2 fused to a C terminal His-tag derived from E. coli has a molecular weight of 26.0 kDa and contains 234 amino acid residues. |
| Protein Sequence | MKLNSIKSSL GGETPGQAAN RSAGMYQGLA FGGSKKGKNL GQAYPCSSDK ECEVGRYCHS PHQGSSACMV CRRKKKRCHR DGMCCPSTRC NNGICIPVTE SILTPHIPAL DGTRHRDRNH GHYSNHDLGW QNLGRPHTKM SHIKGHEGDP CLRSSDCIEG FCCARHFWTK ICKPVLHQGE VCTKQRKKGS HGLEIFQRCD CAKGLSCKVW KDATYSSKAR LHVCQKITRL EHHHHHH |
| Uniprot ID | O94907 |
| Protein RefSeq | NP_036374.1 |
| mRNA RefSeq | NM_012242.2 |
Figures
SDS-PAGE analysis of recombinant human DKK-2. Sample was loaded in 12.5% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
Reference
- Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice. Naoyuki Kawao et al., Int J Mol Sci. 2020 Apr; 21(7): 2547.
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