Human Dkk-1
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| Cat-Nr. | 200-013 |
| Size | 20 µg |
| Price | 185 € |
| Source | E. coli |
| Label | His-Tag |
| Formulation | lyophilized from PBS |
| Purity Confirmation | 95% by SDS-PAGE & visualized Coomassie stain |
| Length [aa] | 242 |
| Molecular Weight | 26.0 kDa |
| N Terminal Sequence | TNLSV |
| Species Reactivity | Human |
| Buffer | PBS |
| Synonyms | Dickkopf-related protein-1, Dickkopf-1, SK |
| Description | DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 fused to a C terminal His-tag derived from E. coli is a 26 kDa protein containing 235 amino-acid residues. |
| Protein Sequence | TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRLEHHHH HH |
| Uniprot ID | O94907 |
| Protein RefSeq | NP_036374.1 |
| mRNA RefSeq | NM_012242.2 |
Figures
SDS-PAGE analysis of recombinant human DKK-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
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