Human IL-2
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| Cat-Nr. | 200-010 |
| Size | 50 µg |
| Price | 180 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE |
| Length [aa] | 156 |
| Molecular Weight | 15.5 kDa |
| N Terminal Sequence | APTSSSTK and MAPTSSST |
| Endotoxin Levels | < 0.1 ng per µg of IL-2 |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml. |
| Species Reactivity | Human |
| Buffer | 50mM acetic acid |
| Reconstitution | The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. |
| Stability and Storage | The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Synonyms | IL2; IL-2; TCGF; lymphokine |
| Description | IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. |
| Protein Sequence | MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ |
| Uniprot ID | P60568 |
| Protein RefSeq | NP_000577 |
| mRNA RefSeq | NM_000586 |
Figures
SDS-PAGE analysis of recombinant human IL-2. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and visualized with Silver staining.
Dose-dependent stimulation of cell proliferation in D10.G4.1 cells by recombinant human IL-2. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
Reference
- Celecoxib increases lung cancer cell lysis by lymphokine-activated killer cells via upregulation of ICAM-1. M. Schellhorn et al., Oncotarget. 2015 Nov 17; 6(36): 39342–39356.
- Cannabinoids increase lung cancer cell lysis by lymphokine-activated killer cells via upregulation of ICAM-1. M. Haustein et al., Biochem Pharmacol. 2014 Nov 15;92(2):312-25.
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