Human IL-35
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| Cat-Nr. | 100-164 |
| Size | 10 µg |
| Price | 199 € |
| Source | HEK 293 cells |
| Formulation | lyophilized |
| Purity Confirmation | ≥ 90% by SDS-PAGE gel and HPLC analyses. |
| Length [aa] | 406 |
| Molecular Weight | 45.8 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Measured by its ability to inhibit proliferative activity of PBMCs. |
| Species Reactivity | Human, Mouse |
| Synonyms | Interleukin-35 |
| Description | Interleukin-35 is a glycosylated, heterodimeric protein consisting of the p35 subunit from IL-12 (IL-12α) and the β subunit from IL-27 (EBI3). IL-35 can be expressed by regulatory T-cells (Tregs), macrophages, and certain trophoblast and dendritic cells. It is induced in response to inflammation, and generally acts as an inflammation suppressor. IL-35 suppresses inflammation by exerting multiple activities, including the induction of regulatory T-cells and the suppression of Th17 cells. Recombinant Human IL-35 produced from HEK293 cells is a glycosylated protein, primarily heterodimeric, that migrates as a diffuse band centered at an apparent molecular weight of about 60 kDa by SDS-PAGE analysis under non-reducing conditions. Recombinant Human IL-35 expressed in HEK293 cells contains 406 amino acid residues and has a calculated molecular weight of 45.8 kDa. |
| Protein Sequence | α Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS β Subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK |
| Uniprot ID | Alpha: P29459 Beta: Q14213 |
| Protein RefSeq | Alpha: NP_001384921 Beta: NP_005746 |
| mRNA RefSeq | Alpha: NM_001397992 Beta: NM_005755 |
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