Rabbit Anti-Mouse VEGFR-2/Flk-1 (Peptide), soluble
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| Cat-Nr. | 103-PA19 |
| Size | 200 µg |
| Price | 305 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunised with a peptide of the C-terminal end of native mouse soluble VEGFR-2/Flk-1 (GMEASLGDRIAMP). Anti-human soluble VEGFR-2/KDR was purified by affinity chromatography with immobilized Protein A. |
| Antigen | peptide: GMEASLGDRIAMP |
| Application | WB |
| Synonyms | soluble vascular endothelial growth factor receptor-2 ; sKdr; sLy73; sFlk-1; sVEGFR2; sVEGF receptor 2; skinase insert domain protein receptor |
| Description | The antibody recognizes solely the endogenous soluble form of mouse vascular endothelial growth factor receptor 2, alos known as CD309, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. The endogenous soluble mouse esFlk-1 generated by alternative splicing consists of the first 6 Ig-like loops followed by the unique C-terminal end: GMEASLGDRIAMP. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF-A or fully processed VEGF-C; ligand binding plays a key role in vascular development and vascular permeability. |
| Uniprot ID | P35918 |
| Protein RefSeq | ACJ66293.1 |
| mRNA RefSeq | EU884114 |
Figures
Western Blot Analysis: Recombinant mouse endogenous soluble VEGFR-2/Flk-1 (esFlk-1) was produced in insect cells. Western blot was performed using our mab against mouse esFlk-1 recognizing the soluble as well as the transmembrane form of Flk-1 and our new poyclonal antibody directed against the unique C-terminal end of the endogenous esFlk-1 (GMEASLGDRIAMP) recognizing solely the endogenous form. There is no cross reactivity of both antibodies with human esKDR. The endogenous esFlk-1 generated by alternative splicing consists of the first 6 Ig-like loops.
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