Rabbit Anti-Human ESAM
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| Cat-Nr. | 102-PA42 |
| Size | 200 µg |
| Price | 305 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human soluble ESAM (Endothelial cell-selective adhesion molecule; Q30-A247; SwissProt: #Q96AP7) as the immunizing antigen. Anti-human ESAM was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant human ESAM (RT #300-057) |
| Application | WB, IF, FC |
| Synonyms | ESAM; W117m |
| Description | Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V type and one C2 type Ig domain and is involved in homophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI. The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cellto cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2. |
| Uniprot ID | Q96AP7 |
| Protein RefSeq | NP_620411.2 |
| mRNA RefSeq | NM_138961.2 |
Figures
Western Blot analysis of anti-human ESAM. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Immunofluorescence staining of vascular endothelial cells from human foreskin (cryo-section of unfixed tissue) with anti-human ESAM (green/red).
Specimen provided by Prof. Dr. J. Wilting and Dr. K. Buttler, Göttingen.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
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