Rabbit Anti-Human Dkk-1
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| Cat-Nr. | 102-PA33S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Purified from sera of rabbits immunized with highly pure recombinant human DKK1 produced in E. coli. |
| Antigen | recombinant human Dkk-1 |
| Application | WB |
| Synonyms | Dickkopf-related protein-1, Dickkopf-1, SK |
| Description | DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 fused to a C terminal His-tag derived from E. coli is a 26 kDa protein containing 235 amino-acid residues. |
| Uniprot ID | O94907 |
| Protein RefSeq | NP_036374.1 |
| mRNA RefSeq | NM_012242.2 |
Figures
Western Blot analysis with recombinant human DKK-1. Samples were loaded in 12,5% SDS-polyacrylamide gel under reducing conditions.
Immunofluorescence staining of human foreskin (cryo-section of PFA-fixed tissue) with anti-human DKK-1 (green; 5µg/ml). Nuclei counter-stained with Dapi (blue).
Specimen provided by Prof. Dr. J. Wilting and Dr. K. Buttler, Göttingen.
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
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