Rabbit Anti-Human CCM-3
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| Cat-Nr. | 102-PA27S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | 0.5X PBS, pH 7.2 |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human CCM3 (RT #300-056) |
| Application | WB, IF |
| Synonyms | PDCD10; CCM3; TFAR15; programmed cell death 10 |
| Description | Cerebral cavernous malformations (CCMs) are sporadically acquired or inherited vascular lesions of the central nervous system consisting of clusters of dilated thin-walled blood vessels that predispose individuals to seizures and stroke. Mutations in CCM1, CCM2, or CCM3 lead to cerebral cavernous malformations, one of the most common hereditary vascular diseases of the brain. Endothelial cells within these lesions are the main disease compartments. Here, we show that adenoviral CCM3 expression inhibits endothelial cell migration, proliferation, and tube formation while down regulation of endogenous CCM3 results in increased formation of tube-like structures. Adenoviral CCM3 expression does not induce apoptosis under normal endothelial cell culture conditions but protects endothelial cells from staurosporine-induced cell death. Tyrosine kinase activity profiling suggests that CCM3 supports PDPK-1/Akt-mediated endothelial cell quiescence and survival (Schleider et al, Neurogenetics 12, 2011). |
| Uniprot ID | Q9BUL8 |
| Protein RefSeq | NP_009148.2 |
| mRNA RefSeq | NM_007217.3 |
Figures
Immunofluorescence staining (green) of human foreskin (cryo-section of unfixed tissue) with anti-human CCM3 (K5548; dilution 1:50) [Cat# 102-PA27]. A) Note specific staining in the epidermis and in the wall of microvessels. B) Negative control of a consecutive section. Nuclei counter-stained with Dapi (blue). Specimen provided by Prof. Dr. J. Wilting, Goettingen.
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Western Blot analysis of anti-human CCM-3.
The experiment was performed by Elisabetta Dejana’s group, IFOM-IEO-Campus, Milan Italy
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