Rabbit Anti-Human CoupTF2/NR2F2
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| Cat-Nr. | 102-PA17S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | recombinant human CoupTFII (Met1-Pro190) |
| Synonyms | COUP-TF2, Apolipoprotein A-I regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2 |
| Description | Tumor growth depends on nutrients and oxygen supplied by the vasculature through angiogenesis. It was shown that the chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), a member of the nuclear receptor family, is a major angiogenesis regulator within the tumor microenvironment. COUP-TFs consist of two highly homologous subtypes, COUP-TFI (EAR-3, NR2F1) and COUP-TFII (ARP-1, NR2F2). Conditional ablation of COUP-TFII in the tumor microenvironment severely compromised neoangiogenesis and lymphangiogenesis during pancreatic tumor progression and metastasis. It was shown that COUP-TFII plays a cell-autonomous role in endothelial cells to control blood vessel sprouting by regulating cell proliferation and migration. Mechanistic investigations revealed that COUP-TFII suppressed vascular endothelial growth factor receptor-2 (VEGFR-2/KDR) signaling by transcriptionally repressing the expression of VEGFR-1, thereby curtailing a central angiogenic driver of vascular growth. These results implicate COUP-TFII as a critical factor in tumor angiogenesis through regulation of VEGF/VEGFR-2 signaling, suggesting COUP-TFII as a candidate target for antiangiogenic therapy. |
| Uniprot ID | P24468 |
| Protein RefSeq | NP_066285.1. |
| mRNA RefSeq | NM_021005.3. |
Figures
Western Blot analysis of anti-human CoupTF2 (fragment). Samples were loaded in 15% SDS-PAGE under reducing conditions. As detection system an AP-conjugated secondary antibody was used.
Immunofluorescence staining of human placenta (fixed for 30 min in 4% PFA) with anti-human CoupTFII [Cat# 102-PA17]. A) Signal (red) in stromal cells of the placenta; B) Control without primary antibody shows no signal. Bar = 60µm
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
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