Rabbit Anti-Human FABP5
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| Cat-Nr. | 102-PA142S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human FABP5. |
| Antigen | Recombinant human FABP5 (RT #400-024) |
| Application | WB |
| Synonyms | Epidermal-type fatty acid-binding protein, E-FABP, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog, PA-FABP |
| Description | Human FABP5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively. |
| Uniprot ID | Q01469 |
| Protein RefSeq | NP_001435 |
| mRNA RefSeq | NM_001444 |
Figures
Western Blot Analysis of recombinant human FABP5 using a rabbit anti-human FABP5 polyclonal antibody [Cat# 102-PA142]. (WB: AP-conjugated secondary antibody)
Double IF staining of human FABP5 (green) and CD31 (red) in a coculture of HDLEC and NIH3T3 cells with a polyclonal rabbit anti-human FABP5 antibody [Cat# 102-PA142] and a monoclonal mouse anti-human CD31 antibody [Cat# 101-M92]. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour 488 (1:600) [Dianova], goat anti-mouse PE (1:400) [Santa Cruz].
Immunofluorescence staining (green) of cryo-sections of human foreskin (fixed 15 min in 4% PFA) with anti-human FABP5 (5µg/ml) [Cat# 102-PA142] and counter staining of nuclei with Dapi (upper panel) and control (lower panel).
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
Western Blot analysis of recombinant human FABP4 [Cat# 400-018] and human FABP5 [Cat# 400-024] using a rabbit polyclonal anti-human FABP5 antibody [Cat# 102-PA142]. There is weak cross reactivity with FABP4 visible. [WB: AP-conjugated secondary antibody]
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