Rabbit Anti-Human Wnt-3a
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| Cat-Nr. | 102-PA140 |
| Size | 200 µg |
| Price | 305 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human Wnt-3a |
| Application | WB |
| Synonyms | Wnt-3a |
| Description | Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation. Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues. |
| Uniprot ID | P56704 |
| Protein RefSeq | NP_149122 |
| mRNA RefSeq | NM_033131 |
Figures
Western Blot analysis of recombinant human Wnt3a [Cat# 400-022] using an anti-human Wnt3a polyclonal antibody.
Western Blot analysis of recombinant human and mouse Wnt3a [Cat# 400-022; M30-231] using a rabbit anti-human Wnt3a antibody [Cat# 102-PA140] directed against recombinant human Wnt3a. As expected there is a cross reactivity with mouse Wnt3a. As secondary antibody an AP-conjugated goat anti-rabbit antibody was used.
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