Rabbit Anti-Human VEGF-A
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| Cat-Nr. | 102-PA02 |
| Size | 200 µg |
| Price | 240 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human VEGF165. Anti-human VEGF-A was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant human VEGF165 (RT #300-036) |
| Application | WB, IP |
| Synonyms | VEGFA; VPF; VEGF; MVCD1 |
| Description | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165). All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. The antibody is against the N-terminus and will recognize all VEGF-A isoforms. |
| Uniprot ID | P15692 |
| Protein RefSeq | NP_001165097 |
| mRNA RefSeq | NM_001171626 |
Figures
Western Blot Analysis of recombinant human / mouse VEGF-A and PlGF-1 using a polyclonal antibody directed against recombinant human VEGF165 produced in insect cells. The recognition of the unreduced proteins is weaker. There is a clear cross reactivity with mouse VEGF-A visible.
Reference
- Reduction of circulating soluble Flt-1 alleviates preeclampsia-like symptoms in a mouse model. A. Bergmann et al., J Cell Mol Med. 2010 Jun; 14(6b): 1857–1867.
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