E. coli Anti-Human FGFR-3 neutralizing antibody
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| Cat-Nr. | 500-002 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | Recombinant IG |
| Isotype | Fab-Fragment |
| Species Reactivity | human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | Lyophilized samples are stable for up to a year at -20°C. Upon reconstitution, the Fab is stable for up to 1 month at 4°C or up to 1 year at -20°C. |
| Synonyms | FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX |
| Description | Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfateproteoglycans as cofactors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF1, 2, 4, 8b, 8e, 8f, 9, and 17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development. |
| Uniprot ID | P22607 |
| Protein RefSeq | NP_000133 |
| mRNA RefSeq | NM_000142 |
Figures
Specific binding and inhibition of FGFR-3-dependent cell proliferation by anti-FGFR-3.
(A) FDCP-FGFR-1, -2, -3 or parental FDCP cells were incubated with anti-FGFR-3 Fab. The cells were stained with Avidin Alexa Fluor 488 and analyzed by FACS;
(B) FDCP cells stably expressing either FGFR-1, FGFR2 or FGFR3 were incubated with increasing amount of anti-FGFR-3 antibody. Two days later, cell proliferation was measured by XTT analysis. (inset) Serum-starved FDCP-FGFR3 cells preincubated with 20 µg/ml antiFGFR-3 were stimulated 5 minutes with FGF. Cell lysates were analyzed by Western with anti-phospho-MAPK.
Functional ELISA: Recombinant human FGF9 [2µg/ml] was coated to a 96-well plate. The single chain antibody sc-FGFR-3 [10µg/ml], the sFGFR-3/Fc [1µg/ml] and both factors together were added. The single chain antibody sc-FGFR-3 nearly completely inhibited the binding of sFGFR-3/Fc to FGF9.
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