Rat Anti-Mouse Sonic Hedgehog
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| Cat-Nr. | 103-M476 |
| Size | 100 µg |
| Price | 380 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#6K12) |
| Isotype | IgG2 |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of Shh (N-terminal). |
| Antigen | recombinant mouse Shh (N-terminal) fragment |
| Application | WB, IHC (F), N |
| Synonyms | Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik |
| Description | Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency. |
| Uniprot ID | Q62226 |
| Protein RefSeq | NP_033196.1 |
| mRNA RefSeq | NM_009170.3 |
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