Rat Anti-Mouse P-Selectin
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| Cat-Nr. | 103-M457 |
| Size | 100 µg |
| Price | 380 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#17H23) |
| Isotype | IgG2 |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of P-Selectin EC domain. |
| Antigen | recombinant mouse P-Selectin extracellular domain |
| Application | WB |
| Synonyms | Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140 |
| Description | Mouse -P-Selectin (GMP140, LECAM3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P- Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or WeibelPalade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL 1 (PSelectin glycoprotein ligand1). PSelectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with LSelectin, PSelectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving ESelectin, and later ICAM1 and VCAM1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2t-erminal C-type lectin domain and an EGF like domain followedby a series of of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology. |
| Uniprot ID | Q01102 |
| Protein RefSeq | NP_035477.1 |
| mRNA RefSeq | NM_011347.2 |
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