Rat Anti-Mouse EphA3
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| Cat-Nr. | 103-M164 |
| Size | 100 µg |
| Price | 380 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#7C13) |
| Isotype | IgG2 |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with recombinant mouse EphA3 extracellular domain. IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | Recombinant mouse EphA3 extracellular domain |
| Application | WB |
| Synonyms | Epha3; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086 |
| Description | EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA3 has been shown to bind ephrinA5, ephrinA2, ephrinA3, ephrinA1, ephrinA4, and ephrinB1. The extracellular domains of mouse and human EphA3 share greater than 96% amino acid identity. Only membranebound or Fcclustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis. |
| Uniprot ID | Q8BRB1 |
| Protein RefSeq | NP_034270.1 |
| mRNA RefSeq | NM_010140.3 |
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