Mouse Anti-Human VEGF-C
Slide this table
| Cat-Nr. | 101-M90 |
| Size | 100 µg |
| Price | 255 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#9E7) |
| Isotype | IgG2a |
| Species Reactivity | Human, rat |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human VEGF-C recombinant protein). The IgG2a fraction of culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Recombinant human VEGF-C (RT# 300-079) |
| Application | WB, IHC (F) |
| Synonyms | vascular endothelial growth factor C; VEGFC; VRP; Flt4-L |
| Description | VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions. |
| Uniprot ID | P49767 |
| Protein RefSeq | NP_005420.1 |
| mRNA RefSeq | NM_005429.2 |
Figures
Staining of VEGF-C on cryo sections (acetone-fixed) of rat mammary tumors.
Staining of VEGF-C on cryo sections (unfixed) of rat mammary tumors.
IHC with paraffin-embedded sections of human colon carcinoma tissue.
Provided by Wroclaw Medical University Department of Histology and Embryology.
All prices plus VAT + possible delivery charges