Mouse Anti-Human VEGFR-3/FLT-4
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| Cat-Nr. | 101-M36 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#9D9) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (recombinant human VEGFR-3/FLT-4) extracellular domain. |
| Antigen | recombinant human soluble VEGFR-3/FLT-4 |
| Application | ELISA, WB, IHC |
| Synonyms | vascular endothelial growth factor receptor-3; FLT4; PCL; LMPH1A; VEGFR3; fms-related tyrosine kinase 4 |
| Description | VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis. |
| Uniprot ID | P35916 |
| Protein RefSeq | NP_002011 |
| mRNA RefSeq | NM_002020 |
Figures
Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 #9D9 [Cat# 101-M36] and polyclonal rabbit anti-human FLT-4 (D) [Cat# 102-PA22]: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4.
The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria
FACS analysis with HDMVEC and HUVEC.
Western analysis of recombinant human soluble VEGFR-3/FLT-4.
FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Reference
- Absence of lymphatic vessels in term placenta. J. Becker et al., BMC Pregnancy Childbirth. 2020; 20: 380.
- Molecular and Cellular Effects of In Vitro Shockwave Treatment on Lymphatic Endothelial Cells. S. Rohringer et al., PLoS One. 2014; 9(12): e114806.
- Effective suppression of vascular network formation by combination of antibodies blocking VEGFR ligand binding and receptor dimerization. D. Tvorogov et al., Cancer Cell. 2010 Dec 14;18(6):630-40.
- Similarities and differences of human and experimental mouse lymphangiomas. Kasten P. et al., Dev Dyn. 2007 Oct;236(10):2952-61.
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