Mouse Anti-Human VEGFR-1/Flt-1
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| Cat-Nr. | 101-M26 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#EIC) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 D1-D5 (Cat.-No: S01-011) as the immunizing antigen. Mouse IgG1 antibody (clone Flt-1/EIC) from hybridomas was purified from cell culture supernatant by Protein G chromatography. The monoclonal antibody will detect native and denatured human VEGFR-1/Flt-1 in ELISA experiments, immunocytochemistry and cell sorting. |
| Antigen | Recombinant human soluble Flt-1 (D5) |
| Application | ELISA, WB, IP, FC |
| Synonyms | vascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1 |
| Description | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. |
| Uniprot ID | P17948 |
| Protein RefSeq | NP_001153392 |
| mRNA RefSeq | NM_001159920 |
Figures
Western analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is no cross reactivity with mouse sFlt-1(D7)-Fc as well as with human and mouse sKDR and human sFLT-4 visible.
FACS analysis with porcine aortic endothelial cells (PAE/Flt1) expressing human VEGFR-1/Flt-1.
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