Mouse Anti-Human Podocalyxin
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| Cat-Nr. | 101-M142 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#GB6D9C11) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen. The mouse IgG1 antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | membrane protein fraction of isolated human glomeruli |
| Application | IHC (P); IF |
| Synonyms | PODXL; PC; PCLP; Gp200; PCLP-1; Podocalyxin-like protein, GCTM-2 antigen, Gp200 |
| Description | Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. It was shown that endothelial cell membranes contain Podocalyxin. By immunoelectron microscopy Podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of Podocalyxin was confirmed in SDS extracts of lung tissue by immunoblotting. It is concluded that (a) Podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface. |
| Uniprot ID | O00592 |
| Protein RefSeq | NP_001018121 |
| mRNA RefSeq | NM_001018111 |
Figures
IHC staining of the podocytes in the glomerulus and the tubule-interstitial blood vessels (paraffin-embedded tissue-sections fixed in formalin) with anti-human Podocalyxin [Cat# 101-M142].
Immunofluorescence staining of the podocytes in the glomerulus and the tubule-interstitial blood vessels (paraffin-embedded tissue-sections fixed in formalin) with anti-human Podocalyxin [Cat# 101-M142].
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