Mouse Anti-Human E-Selectin
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| Cat-Nr. | 101-M129 |
| Size | 100 µg |
| Price | 380 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#9E7) |
| Isotype | IgG2 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant E-selectin protein. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recombinant human E-Selectin |
| Application | WB, IHC (P) |
| Synonyms | SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2 |
| Description | E-Selectin (Endothelial Leukocyte Adhesion Molecule1, ELAM1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL 1β and TNFα, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. |
| Uniprot ID | P16581 |
| Protein RefSeq | NP_000441.2 |
| mRNA RefSeq | NM_000450 |
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