Description / Podoplanin
Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG |
| Biological Activity | Western Blot: use at 1-5 µg/ml |
| Clone Nr. | (#LF3(B7)D5B3) |
| Species Reactivity | Rat |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated rat glomeruli as the immunizing antigen. The mouse IgG antibody (#LF3(B7)D5B3) from hybridomas was purified from cell culture supernatant by Pro |
| Antigen | membrane protein fraction of isolated rat glomeruli |
| Application | WB, IHC |
| Synonyms | Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha |
| Uniprot ID | Q64294 |
| Protein RefSeq | NP_062231.1 |
| mRNA RefSeq | NM_019358.1 |