Description / PCPE-1
Procollagen Cendopeptidase enhancers, known as PCPEs or PCOLCEs, are secreted extracellular matrix glycoproteins that consist of two CUB domains and one NTR domain. They are known to stimulate enzymatic cleavage of procollagens IIII by the BMP-1/ tolloid family of metallo-proteases, also known as procollagen Cproteinases. PCPE1 is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin. Although BMP1/ tolloid proteinases are involved in processing of multiple extracellular proteins, the enhancer activity of PCPE1 is specific to procollagens since it has no effect on BMP1/ tolloid cleavage of other substrates. It is thought that PCPE1 enhances cleavage of procollagens by binding to the substrate, inducing a conformation change in the substrate, although interaction between PCPE-1 and fulllength BMP-1/ tolloid proteinases has also been reported.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#7H78) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of PCPE-1. |
| Antigen | recombinant mouse PCPE-1 |
| Application | WB |
| Synonyms | Pcolce; P14; Astt2; Astt-2; PCPE-1 |
| Uniprot ID | Q61398 |
| Protein RefSeq | NP_032814.2 |
| mRNA RefSeq | NM_008788.2 |