Description / Pappalysin-2
Pappalysins belong to a fifth family of metzincin proteases that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins. PAPPA is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the non-pregnant state. PAPPA is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPPA are significantly reduced when a fetus affected by Down syndrome is present. PAPPA cleaves Insulin-like Growth FactorBinding Protein4 and 5 (IGFBP4 and 5) at a single site, resulting in the release of bioactive IGF. Compared to PAPPA, PAPPA2 (also called PAPPE), the second member of the family is less characterized. PAPPA2 shares 45% amino acid identity to PAPPA in the mature form, which is synthesized as a preproprotein consisting of multiple domains. The prepro region (residues 1-233) and the C-terminal region (residues 1397-1791) are not included in recombinant human PAPPA2.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Clone Nr. | (#8M12) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human Pappalysin-2, also called PAPP-A2. |
| Antigen | recombinant human Pappalysin-2 |
| Application | WB |
| Synonyms | PAPPA2; PAPPE; PLAC3; PAPP-A2 |
| Uniprot ID | Q9BXP8 |
| Protein RefSeq | NP_064714.2 |
| mRNA RefSeq | NM_020318.2 |