Description / MCP-5
MCP-5 or CCL12 is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid sequence identity in the mature protein). Mouse MCP-5 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. MCP-5 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, its expression is also induced in activated macrophages and mast cells. The gene for mouse MCP1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant MCP-5 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, MCP-5 will also chemoattract eosinophils. MCP-5 has been found to be a functional ligand for CCR2 but not CCR1, CCR3 or CCR5.
More Information
| Size | 100 µg |
|---|---|
| Source | Rabbit |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant murine MCP-5 (murine MCP-5). Anti-murine MCP-5 specific antibody was purified by affinity chromatography employing immobilized murine MCP-5 matrix. |
| Antigen | Recombinant Mouse MCP-5 |
| Application | ELISA, WB |
| Synonyms | Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5 |
| Uniprot ID | P21844 |
| Protein RefSeq | NP_034910.1 |
| mRNA RefSeq | NM_010780.2 |