Description / Leptin binding protein
Recombinant chicken leptin binding domain (chLBD), is one polypeptide chain containing 208 amino acids and an additional Ala at N-terminus acids. It consists of the cytokine binding domain of leptin receptor (amino acids 420-626 of chicken leptin receptor and having a molecular mass of ~ 24.5 kDa, It was purified by proprietary chromatographic techniques (see Niv-Spector et al. Biochemical Journal (2005) 390:475-484.
More Information
| Size | 50 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Recombinant chLBDL is fully biologically active as evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio. |
| N Terminal Sequence | AIDVNI |
| Purity Confirmation | > 99.0% as determined by Gel filtration and SDS-PAGE gel. |
| Length [aa] | 208 |
| Molecular Weight | 24.5 kDa |
| Species Reactivity | Chicken |
| Formulation | lyophilized |
| Protein Sequence | AIDVNINIKCETDGYLTKMTCRWSANPNALLLGSSLQLKYHMSKIYCSNFPSTPPESEVKECHFQRNHSYECTFQPVFLLSGYTMWIELKHSLGTLESSPTCVVPADVVKPLPPSNIKAEITRNDGLLNVSWTNPVFTNDDLKFQIRYAVNREELTWELYEVLSVPTRSAVIEVQLCVEYIVQIRCRALDGLGYWSNWSRSAYAAVKD |
| Reconstitution | It is recommended to reconstitute the lyophilized chLBD in sterile water at pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Synonyms | Lep; ob; obese |
| Uniprot ID | Q9I8V6 |
| Protein RefSeq | NP_989654.2 |
| mRNA RefSeq | NM_204323.2 |