Description / IL-2 receptor, soluble
Human IL-2 exerts its biological effects via signaling through its receptor system, IL-2R. IL-2 and its receptor (IL-2R) are required for T-cell proliferation and other fundamental functions which are crucial of the immune response. IL-2R consists of 3 noncovalently linked type I transmembrane proteins which are the alpha (p55), beta (p75), and gamma (p65) chains. The IL-2R alpha chain contains an extracellular domain of 219 amino acids, a transmembrane domain of 19 amino acids, and an intracellular domain of 13 amino acids. The secreted extracellular domain of IL-2R alpha (s-IL-2R-a), also called TAC-antigen, is expressed on leukemia cells, lymphoma cells, approximately 10% NK cells, as well as recently activated T and B cells. Recombinant human s-IL-2R-a is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and Mass Spectrometry.
More Information
| Size | 500 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2a, kappa |
| Species Reactivity | Human |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography. |
| Antigen | Recombinant Human sIL-2 Receptor α |
| Application | ELISA, WB, N |
| Synonyms | IL-2 receptor, soluble |
| Uniprot ID | P01589 |
| Protein RefSeq | NP_000408.1 |
| mRNA RefSeq | NM_000417.2 |