Description / human IFN gamma protein
Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
More Information
Size | 100 µg |
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Source | E. coli |
Biological Activity | Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg). |
Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
Length [aa] | 144 |
Molecular Weight | 16.8 kDa |
Species Reactivity | Monkey, Human, Mouse, Leech, Pig, |
Formulation | lyophilized |
Buffer | 10mM Sodium Phosphate, pH 7.4 |
Protein Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ |
Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier p |
Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C. |
Synonyms | IFNG; IFG; IFI |
Uniprot ID | P01579 |
Protein RefSeq | NP_000610.2 |
mRNA RefSeq | NM_000619 |