human GDF-5 (BMP-14/CDMP-1, Animal Free) protein Human 50 µg
Description / human GDF-5 (BMP-14/CDMP-1, Animal Free) protein
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant Human GDF-5 (BMP-14/CDMP-1) is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains.
More Information
| Size | 50 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 µg /ml. |
| Purity Confirmation | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
| Length [aa] | 120 |
| Molecular Weight | 27 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR |
| Synonyms | Growth/Differentiation Factor-5, Bone Morphogenetic Protein-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1) |
| Uniprot ID | P43026 |
| Protein RefSeq | NP_000548 |
| mRNA RefSeq | NM_000557 |