EphB6 Anti-Mouse 100 µg

In stock

Cat-Nr.
103-M174
Size
100 µg
  €533.00

Description / EphB6

EphB6, also known as Mep, is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteinerich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity. The ligands which bind EphB6 are unknown. However, we have observed that the ephrinB1 and ephrinB2 ligands can bind the immobilized receptor in an ELISAtype assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membranebound or Fcclustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis.

More Information

Size 100 µg
Source Rat
Isotype IgG2
Clone Nr. (#9B17)
Species Reactivity Mouse
Formulation lyophilized
Buffer PBS
Reconstitution Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and Storage Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with recombinant mouse EphB6 extracellular domain. IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigen recombinant mouse EphB6 extracellular domain
Application WB, IHC (P)
Synonyms Ephb6; Mep; Cekl
Uniprot ID O08644
Protein RefSeq NP_001139823.1
mRNA RefSeq NM_001146351.1

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