Description / CD25
The biological effects of interleukin 2 (IL-2) are mediated by its binding to a multi-chain cellular receptor complex. The IL-2 receptor (IL-2 R) complex consists of three glycoprotein chains. The alpha chain (IL-2 R alpha /CD25) and beta chain (IL-2 R beta /CD122) act together with the gamma chain (IL-2 R gamma /CD132) to form a high affinity receptor that transduces the signal upon IL-2 binding. Alone, IL-2 R alpha binds IL-2 with low affinity, but is unable to transduce a signal. The alpha/beta combination binds IL-2 with intermediate affinity, but is still unable to transmit a signal inside the cell. The beta/gamma complex has intermediate affinity and is capable of signaling if the concentration of IL-2 is relatively high.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG1 |
| Clone Nr. | (#7G19) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse CD25 protein). IgG1 fraction of the culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Recombinant Mouse CD25 protein |
| Application | IHC, FC |
| Synonyms | CD25; Il2r; Ly-43; interleukin 2 receptor, alpha chain; Il2ra |
| Uniprot ID | P01590 |
| Protein RefSeq | NP_032393.3 |
| mRNA RefSeq | NM_008367.3 |