Description / ALK5
Most cell types express three sizes of receptors for TGFβ. These are designated Type I (53 kDa), Type II (7085 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membranebound and soluble forms, binds TGFβ1, TGFβ2, and TGFβ3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane bound serine/threonine kinase that binds TGFβ1 and TGFβ3 with high affinity and TGFβ2 with a much lower affinity. The Type I receptor, originally known as ALK5 (Activin receptor-like kinase) is also a membranebound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGFβ. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#3A11) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse ALK-5 extracellular domain. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recmobinant mouse ALK5 extracellular domain |
| Application | WB, IHC |
| Synonyms | Tgfbr1; ALK5; Alk-5; TbetaRI; AU017191; TbetaR-I |
| Uniprot ID | Q64729 |
| Protein RefSeq | NP_033396.1 |
| mRNA RefSeq | NM_009370.2 |