Protocols

Protocols from Researchers

The following protocols are from researchers using ReliaTech's antibodies.

Immunohistochemistry (IHC)

Epitope demasking

Demasking of epitopes in paraffin sections

Heat treatments

Proteolytic Treatments

Immunofluorescence (IF)

Immunofluorescence with lymphatic endothelial cells (LECs)

a) Protocol for immunofluorescence with lympathic endothelial cells (LEC) using Lab-Tek II Chamber Slides. As primary antibodies anti-human LYVE-1 (ReliaTech Cat# 102-PA50), anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).

b) Protocol for immunofluorescence with lympathic endothelial cells (LEC) and HUVECs in a cell culture dish. As primary antibodies anti-human Prox-1 (ReliaTech Cat# 102-PA32) and anti-human CD31 were used (PDF, English).

Flow Cytometry

FACS analysis with human macro- / microvascular endothelial cells

a) HUVEC / HDMVEC (German / English)

Bioassays

a) Thymidine incorporation assay for primary human endothelial cells (PDF, English)
b) Thymidine incorporation assay for Balb/c 3T3 cells (German / English)