Rat Carboxypeptidase-B
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| Cat-Nr. | R20-019 |
| Size | 25 µg |
| Price | 199 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | >95% by SDS-PAGE & HPLC analysis |
| Length [aa] | 307 |
| Molecular Weight | 35.1 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Carboxypeptidase-B sequentially cleaves C terminal K and R residues. |
| Species Reactivity | Rat |
| Buffer | 20mM Tris, pH 8.0 + 50mM NaCl |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer (e.g. PBS) containing a carrier protein (e.g. 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | Cpb1; Cpb |
| Description | Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Carboxypeptidase-B sequentially cleaves C terminal K and R residues. Recombinant rat Carboxypeptidase-B is a 35.1 kDa protein consisting of 307 amino acids. |
| Protein Sequence | ASGHSYTKYN NWETIEAWIQ QVATDNPDLV TQSVIGTTFE GRNMYVLKIG KTRPNKPAIF IDCGFHAREW ISPAFCQWFV REAVRTYNQE IHMKQLLDEL DFYVLPVVNI DGYVYTWTKD RMWRKTRSTM AGSSCLGVDP NRNFNAGWCE VGASRSPCSE TYCGPAPESE KETKALADFI RNNLSTIKAY LTIHSYSQMM LYPYSYDYKL PENYEELNAL VKGAAKELAT LHGTKYTYGP GATTIYPAAG GSDDWSYDQG IKYSFTFELR DTGFFGFLLP ESQIRQTCEE TMLAVKYIAN YVREHLY |
| Uniprot ID | P19223 |
| Protein RefSeq | NP_036665.1 |
| mRNA RefSeq | NM_012533.1 |
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