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Rat VEGF-C

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Cat-Nr.R20-015
Size20 µg
Price230 €
SourceInsect cells
Formulationlyophilized
Purity Confirmation> 90% by SDS-PAGE
Length [aa]127
Molecular Weight15.0 - 20.0 kDa
N Terminal SequenceSIDNEWRKTQ AND NEWRKTQCMP
Biological ActivityThe biological activity was determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).
Species ReactivityRat
Buffer50 mM acetic acid
Stabilizer/CarrierBSA (50-fold)
ReconstitutionThe lyophilized VEGF-C is soluble in water and most aqueous buffers. The lyophilized VEGF-C should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.
Stability and StorageLyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Synonymsvascular endothelial growth factor C; Vegfc
DescriptionVEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 127 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in SDS-PAGE under reducing conditions.
Protein SequenceDTVKLAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIHHHHHH
Uniprot IDO35757
Protein RefSeqNP_446105.1
mRNA RefSeqNM_053653.1

Figures

Figure 1.
SDS-PAGE analysis of recombinant rat VEGF-C. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and visualized with Silver stain.
Figure 3.
VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC). HDLECs were stimulated with increasing amounts of recombinant rat VEGF-C.
Figure 2.
Biological activity of recombinant rat VEGF-C measured by its ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC cells expressing VEGFR-3/FLT-4.

Reference

  1. Targeting Tumor Angiogenesis with the Selective VEGFR-3 Inhibitor EVT801 in Combination with Cancer Immunotherapy. M. R. Paillasse et al., Cancer Res Commun. 2022 Nov; 2(11): 1504–1519.
  2. Atypical cadherin FAT4 orchestrates lymphatic endothelial cell polarity in response to flow. K. L. Betterman et al., J Clin Invest. 2020 Jun 1; 130(6): 3315–3328.
  3. Vascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans. J. Han et al., Cell Rep. 2015 Feb 24; 10(7): 1158–1172.
  4. VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment. L. Schindewolffs et al., Immun Inflamm Dis. 2014 Nov; 2(3): 152–161.
  5. The Indolinone MAZ51 Induces Cell Rounding and G2/M Cell Cycle Arrest in Glioma Cells without the Inhibition of VEGFR-3 Phosphorylation: Involvement of the RhoA and Akt/GSK3β Signaling Pathways. Joo-Hee Park et al., PLoS One. 2014; 9(9): e109055.
  6. A Global Transcriptome Analysis Reveals Molecular Hallmarks of Neural Stem Cell Death, Survival, and Differentiation in Response to Partial FGF-2 and EGF Deprivation. V. Nieto-Estévez et al., PLoS One. 2013; 8(1): e53594.
  7. Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis. C.-F. Calvo et al., Genes Dev. 2011 Apr 15; 25(8): 831–844.
  8. Effects of VEGFR-3 phosphorylation inhibitor on lymph node metastasis in an orthotopic diffuse-type gastric carcinoma model. M. Yashiro et al., Br J Cancer. 2009 Oct 6; 101(7): 1100–1106.
  9. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. B. Le Bras et al., Nat Neurosci. 2006 Mar;9(3):340-8.
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