Mouse Wnt-5a
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| Cat-Nr. | M30-023 |
| Size | 20 µg |
| Price | 310 € |
| Source | E. coli |
| Label | His-Tag |
| Formulation | lyophilized |
| Purity Confirmation | > 90% by SDS-PAGE & Coomassie stain |
| Length [aa] | 328 |
| Molecular Weight | 36.9 kDa |
| N Terminal Sequence | MIIGA |
| Species Reactivity | Mouse |
| Buffer | 50 mM acetic acid |
| Reconstitution | Mouse Wnt5a should be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C. |
| Stability and Storage | The lyophilized mouse Wnt5a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt5a should be stored in working aliquots at -20°C. |
| Synonyms | Wnt-5a; Wnt5a; Wnt 5a; Wnt |
| Description | Wnt-5a, a member of the highly conserved Wnt protein family, is a 352 amino acid protein containing 23 conserved cysteines and contains a cysteine modified by palmitate which is essential for receptor binding and biological activity. Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signaling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterized relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers. |
| Protein Sequence | MIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEIVDQFVCK LEHHHHHH |
| Uniprot ID | P22725 |
| Protein RefSeq | NP_033550.2 |
| mRNA RefSeq | NM_009524.3 |
Figures
SDS-PAGE analysis of recombinant mouse Wnt5a. Sample was loaded in 12.5% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
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