Mouse Wnt-3a
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| Cat-Nr. | M10-231S |
| Size | 2 µg |
| Price | 95 € |
| Source | Cell culture |
| Formulation | lyophilized |
| Purity Confirmation | > 75% by SDS-PAGE & HPLC analysis |
| Length [aa] | 334 |
| Molecular Weight | 38-41 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6 ng/ml. |
| Species Reactivity | Human, Mouse |
| Synonyms | Wnt3a; vt; Wnt-3a |
| Description | Wnt-3a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Recombinant murine Wnt-3a is a monomeric glycoprotein containing 334 amino acid residues. |
| Protein Sequence | SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK |
| Uniprot ID | P27467 |
| Protein RefSeq | NP_033548 |
| mRNA RefSeq | NM_009522 |
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