Mouse FGF-7/KGF
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| Cat-Nr. | M10-042 |
| Size | 10 µg |
| Price | 199 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | ≥ 95% by SDS-PAGE gel and HPLC analyses |
| Length [aa] | 164 |
| Molecular Weight | 18.9 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-5.0 ng/ml. |
| Species Reactivity | Mouse |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7 |
| Description | FGF‐7/KGF is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF‐7/KGF is a mitogen factor specific for epithelial cells and keratinocytes. FGF‐7/KGF signals through FGFR 2b. FGF‐7/KGF plays a role in kidney and lung development, as well as in angiogenesis and wound healing. Recombinant Murine FGF‐7/KGF is an 18.9 kDa protein consisting of 164 amino acid residues. |
| Protein Sequence | MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT |
| Uniprot ID | P36363 |
| Protein RefSeq | NP_032034.1 |
| mRNA RefSeq | NM_008008.4 |
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