Mouse IL-6
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| Cat-Nr. | M10-017 |
| Size | 20 µg |
| Price | 185 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & Coomassie stain |
| Length [aa] | 188 |
| Molecular Weight | 21.7 kDa |
| N Terminal Sequence | MFPTS |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of murine hybridoma B9 cells is in the range of 5-20 pg/ml. |
| Species Reactivity | Monkey, Mouse, Rat, Trout |
| Buffer | 50 mM Acetic Acid |
| Synonyms | Il6; Il-6 |
| Description | IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 187 amino acid residues. |
| Protein Sequence | MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
| Uniprot ID | P08505 |
| Protein RefSeq | NP_112445.1 |
| mRNA RefSeq | NM_031168 |
Figures
SDS-PAGE analysis of recombinant mouse IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Proliferation assay with mouse hybridoma cell line B9. The cells were stimulated with increasing amounts of recombinant mouse IL-6.
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