Mouse IL-3
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| Cat-Nr. | M10-015 |
| Size | 10 µg |
| Price | 185 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & Coomassie stain |
| Length [aa] | 140 |
| Molecular Weight | 15.8 kDa |
| N Terminal Sequence | ASISGR |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is in the range of 20-100 pg/ml. |
| Species Reactivity | Rat, Mouse, Human, Monkey, Bacteria, Gerbil |
| Buffer | PBS |
| Synonyms | Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu |
| Description | IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. |
| Protein Sequence | ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT SRPPQPASGS VSPNRGTVEC |
| Uniprot ID | P01586 |
| Protein RefSeq | NP_034686.2 |
| mRNA RefSeq | NM_010556.4 |
Figures
SDS-PAGE analysis of recombinant mouse IL-3. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Proliferation assay with the mouse cell line NFS60. The cells were stimulated with increasing amounts of recombinant mouse IL-3.
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