Zebrafish Growth Hormone mutant (G119R)

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Cat-Nr.500-023S
Size10 µg
Price190 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 99.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Length [aa]186
Molecular Weight21.1 kDa
N Terminal SequenceAQRLFN
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityThe biologically activity of zfGH mutant in PDF-P1 3B9 cells stably transfected with rabbit GH receptors has not yet been tested.
ReconstitutionIt is recommended to reconstitute the lyophilized zfGH mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionRecombinant zebrafish growth hormone mutant G119R (zbGH mutant) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton.
Protein SequenceAQRLFNNAVIRVQHLHQLAAKMINDFEEGLMPEERRQLSKIFPLSFCNSDSIETPTGKDETQKSSMLKLLRISFRLIESWEFPSQTLSSTISNSLTIGNPNQITEKLADLKMGISVLIKRCLDGQPNMDDNDSLPLPFEDFYLTVGETSLRESFRLLACFKKDMHKVETYLRVANCRRSLDSNCTL
Uniprot IDQ1JQ34
Protein RefSeqNP_001018328.2
mRNA RefSeqNM_001020492.2

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