Human Wnt-5a
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| Cat-Nr. | 400-023 |
| Size | 25 µg |
| Price | 170 € |
| Source | E. coli |
| Label | His-Tag |
| Formulation | lyophilized |
| Purity Confirmation | > 90% by SDS-PAGE & Coomassie stain |
| Length [aa] | 328 |
| Molecular Weight | 36.9 kDa |
| N Terminal Sequence | MIIGA |
| Species Reactivity | Human |
| Buffer | 50 mM acetic acid |
| Reconstitution | Human Wnt5a should be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C. |
| Stability and Storage | The lyophilized human Wnt5a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt5a should be stored in working aliquots at -20°C. |
| Synonyms | Wnt-5a; Wnt5a; Wnt 5a; Wnt |
| Description | Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signalling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterised relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers. |
| Protein Sequence | MIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCKLEHHHHHH |
| Uniprot ID | P41221 |
| Protein RefSeq | NP_003383.2 |
| mRNA RefSeq | NM_003392.4 |
Figures
SDS-PAGE analysis of recombinant human Wnt5a. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
Binding of recombinant human Wnt5a [Cat# 400-023] to recombinant human sROR2 [Cat# S01-074] in a functional ELISA. sROR2 was coated with 2µg/ml (100µg/well) and increasing amounts of Wnt5a was added. Detection was performed using a polyclonal rabbit anti-human Wnt5a and a goat anti-rabbit Biotin conjugated secondary antibody.
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