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Human VEGF-C

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Cat-Nr.300-078
Size5 µg
Price80 €
SourceInsect cells
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 90% by SDS-PAGE
Length [aa]121
Molecular Weight18.0-24.0 kDa
N Terminal SequenceDPTEETI
Biological ActivityThe biological activity was determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).
Species ReactivityHuman
BufferWater
Stabilizer/CarrierBSA (50-fold)
ReconstitutionThe lyophilized VEGF-C is soluble in water and most aqueous buffers. The lyophilized VEGF-C should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.
Stability and StorageLyophilized samples are stable for more than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Synonymsvascular endothelial growth factor C; VEGFC; VRP; Flt4-L; VEGF c
DescriptionVEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 121 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Protein SequenceDPTEETIKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLHHHHHH
Uniprot IDP49767
Protein RefSeqNP_005420.1
mRNA RefSeqNM_005429.2

Figures

Figure 1.
Biological activity measured by its ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC cells expressing VEGFR-3/FLT-4.
Figure 3.
VEGF-C Sandwich-ELISA using recombinant human VEGF-C as standard [Cat# 300-079]. Mouse anti-human VEGF-C #9/G10 (Cat# 101-M88) was used as capture antibody, Biotinylated mouse anti-human VEGF-C #107/A11 (Cat# 101-MBi89) was used for detection.
Figure 2.
VEGF-C-induced proliferation of HDLECs. HDLECs were stimulated with increasing amounts of recombinant human VEGF-C.

Reference

  1. Arsenic Trioxide Decreases Lymphangiogenesis by Inducing Apoptotic Pathways and Inhibition of Important Endothelial Cell Receptors. I. Hrgovic et al., Curr Issues Mol Biol. 2024 Jan; 46(1): 67–80.
  2. Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro. C. Hromada et al., Biomolecules. 2022 Jun; 12(6): 820.
  3. Comparison of avascular lymph node fragment transplantation techniques to optimize lymphangiogenesis in the minipig model. C. Hadamitzky, F. Bruns, KF. Gratz et al., Eur J Plast Surg (2021)
  4. Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis. P. Mazzuca et al., J Virol. 2017 Aug 15; 91(16): e00801-17.
  5. The histone deacetylase inhibitor trichostatin a decreases lymphangiogenesis by inducing apoptosis and cell cycle arrest via p21-dependent pathways. I. Hrgovic et al., BMC Cancer. 2016; 16: 763.
  6. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells. B. Lorusso et al., Biomed Res Int. 2015; 2015: 747864.
  7. Genetic Heterogeneity of Lymphangiogenesis in Different Mouse Strains. B. Regenfuß et al., Am J Pathol. 2010 Jul; 177(1): 501–510.
  8. Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Y. Wang et al., Nature. 2010 May 27;465(7297):483-6.
  9. Alternatively spliced VEGF receptor-2 is an essential endogenous inhibitor of lymphatic vessels. J.C. Romulo et al., Nat Med. Author manuscript; Nat Med. 2009 Sep; 15(9):1023–1030.
  10. U94 of human herpesvirus 6 inhibits in vitro angiogenesis and lymphangiogenesis. A. Caruso et al., Proc Natl Acad Sci U S A. 2009 Dec 1; 106(48): 20446–20451.
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